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glast1 microbeads  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec glast1 microbeads
    Glast1 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 354 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glast1/CD11b+(Microglia)+MicroBeads%2C+human+and+mouse/pm40954280-185-13-15
    Average 96 stars, based on 354 article reviews
    glast1 microbeads - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: Retrotransposon instability dominates the acquired mutation landscape of mouse induced pluripotent stem cells
    Article Snippet: To obtain astrocytes, brain tissue was processed using a MACS Neural Tissue Dissociation Kit (T) (Miltenyi Biotec, Cat#: 130-093-231) and manually dissected according to manufacturer’s instructions. .. Cells were then collected and incubated with antibodies directed against Glast1 (Allophycocyanin-conjugated, ACSA-1, 1:10 dilution) (Miltenyi Biotec, Cat#: 130-098-803), 1:100 dilution of Anti-mouse CD133 antibody conjugated to PE (Thermo Fisher Scientific, Cat#: 12-1331-80), 1:200 dilution of Anti-mouse CD45 antibody conjugated to PE-Cy7 (BD Biosciences, Cat#: 552848) and 1:200 dilution of Anti-mouse CD31 antibody conjugated to PE-Cy7 (Thermo Fisher Scientific, Cat#: 25-0311-82). ..

    Article Title: Retrotransposon instability dominates the acquired mutation landscape of mouse induced pluripotent stem cells.
    Article Snippet: To obtain astrocytes, brain tissue was processed using a MACS Neural Tissue Dissociation Kit (T) (Miltenyi Biotec, Cat#: 130-093-231) andmanually dissected according tomanufacturer’s instructions. .. Cells were then collected and incubated with antibodies directed against Glast1 (Allophycocyanin-conjugated, ACSA-1, 1:10 dilution) (Miltenyi Biotec, Cat#: 130-098-803), 1:100 dilution of Anti-mouse CD133 antibody conjugated to PE (Thermo Fisher Scientific, Cat#: 12-1331-80), 1:200 dilution of Anti-mouse CD45 antibody conjugated to PE-Cy7 (BD Biosciences, Cat#: 552848) and 1:200 dilution of Anti-mouse CD31 antibody conjugated to PE-Cy7 (Thermo Fisher Scientific, Cat#: 25- 0311-82). ..

    Article Title: Retrotransposon instability dominates the acquired mutation landscape of mouse induced pluripotent stem cells
    Article Snippet: To obtain astrocytes, brain tissue was processed using a MACS Neural Tissue Dissociation Kit (T) (Miltenyi Biotec, Cat#: 130-093-231) and manually dissected according to manufacturer’s instructions. .. Cells were then collected and incubated with antibodies directed against Glast1 (Allophycocyanin-conjugated, ACSA-1, 1:10 dilution) (Miltenyi Biotec, Cat#: 130-098-803), 1:100 dilution of Anti-mouse CD133 antibody conjugated to PE (Thermo Fisher Scientific, Cat#: 12-1331-80), 1:200 dilution of Anti-mouse CD45 antibody conjugated to PE-Cy7 (BD Biosciences, Cat#: 552848) and 1:200 dilution of Anti-mouse CD31 antibody conjugated to PE-Cy7 (Thermo Fisher Scientific, Cat#: 25-0311-82). ..

    Flow Cytometry:

    Article Title: Microglia have a more extensive and divergent response to interferon-α compared with astrocytes.
    Article Snippet: Funding information National Health and Medical Research Council project, Grant/Award Number: 512407 Abstract Type I interferons (IFN-I) are crucial for effective antimicrobial defense in the central nervous system (CNS) but also can cause severe neurological disease (termed cerebral interferonopathy) as exemplified by Aicardi-Goutières Syndrome.. In the CNS, microglia and astrocytes have essential roles in host responses to infection and injury, with both cell types responding to IFN-I.. While the IFN-I signaling pathways are the same in astrocytes and microglia, the extent to which the IFN-I responses of these cells differ, if at all, is unknown.

    Software:

    Article Title: Microglia have a more extensive and divergent response to interferon-α compared with astrocytes.
    Article Snippet: Funding information National Health and Medical Research Council project, Grant/Award Number: 512407 Abstract Type I interferons (IFN-I) are crucial for effective antimicrobial defense in the central nervous system (CNS) but also can cause severe neurological disease (termed cerebral interferonopathy) as exemplified by Aicardi-Goutières Syndrome.. In the CNS, microglia and astrocytes have essential roles in host responses to infection and injury, with both cell types responding to IFN-I.. While the IFN-I signaling pathways are the same in astrocytes and microglia, the extent to which the IFN-I responses of these cells differ, if at all, is unknown.



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    Fig. 4. Unique effects of TLR4 and TLR1/2 ligands on brainstem astrocytes, microglia and neurons. A. Schematic overview of cell isolation and magnetic sorting strategy for separation of CD-11 microglia, <t>GLAST1</t> + astrocytes and neurons (see methods for details). A1-A3. Immunolabelling for astrocytes, microglia and neurons. Astrocytes were labeled for GLAST1, ALDH1L1 and GFAP. Microglia cells were labeled for IBA1, CD45 and TNFa. Neurons were labeled for NeuN, MAP2, TUJ1. B. Principal component analysis showing Kmeans clusters of DEGs in LPS and PAM3CSK4 related to control obtained from sample signals for each group. C-E. Volcano plots showing DEGs of isolated astrocytes(C), microglia (D), and neurons (E), respectively, in LPS related to control. F-H. Volcano plots showing DEGs in isolated astrocytes (F), microglia(G), and neurons (H), respectively, in PAM3CSK4 related to control. I-M. Enrichment analysis of top GOs in astrocytes, microglia and neurons either LPS related to Control or PAM3CSK4 related to control as indicated on top of each graph. N-O. Selected GOs enriched in neurons for neuronal and glial and metabolism processes. Note that we selected GOs of interest that were related to metabolism, neuronal processes and glial processes as indicated on the schematic. All volcano plots are represented with fold change > 2.0 and p-value < 0.05.
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    Image Search Results


    Fig. 4. Unique effects of TLR4 and TLR1/2 ligands on brainstem astrocytes, microglia and neurons. A. Schematic overview of cell isolation and magnetic sorting strategy for separation of CD-11 microglia, GLAST1 + astrocytes and neurons (see methods for details). A1-A3. Immunolabelling for astrocytes, microglia and neurons. Astrocytes were labeled for GLAST1, ALDH1L1 and GFAP. Microglia cells were labeled for IBA1, CD45 and TNFa. Neurons were labeled for NeuN, MAP2, TUJ1. B. Principal component analysis showing Kmeans clusters of DEGs in LPS and PAM3CSK4 related to control obtained from sample signals for each group. C-E. Volcano plots showing DEGs of isolated astrocytes(C), microglia (D), and neurons (E), respectively, in LPS related to control. F-H. Volcano plots showing DEGs in isolated astrocytes (F), microglia(G), and neurons (H), respectively, in PAM3CSK4 related to control. I-M. Enrichment analysis of top GOs in astrocytes, microglia and neurons either LPS related to Control or PAM3CSK4 related to control as indicated on top of each graph. N-O. Selected GOs enriched in neurons for neuronal and glial and metabolism processes. Note that we selected GOs of interest that were related to metabolism, neuronal processes and glial processes as indicated on the schematic. All volcano plots are represented with fold change > 2.0 and p-value < 0.05.

    Journal: Brain, behavior, and immunity

    Article Title: Metabolic trade-offs in Neonatal sepsis triggered by TLR4 and TLR1/2 ligands result in unique dysfunctions in neural breathing circuits.

    doi: 10.1016/j.bbi.2024.03.027

    Figure Lengend Snippet: Fig. 4. Unique effects of TLR4 and TLR1/2 ligands on brainstem astrocytes, microglia and neurons. A. Schematic overview of cell isolation and magnetic sorting strategy for separation of CD-11 microglia, GLAST1 + astrocytes and neurons (see methods for details). A1-A3. Immunolabelling for astrocytes, microglia and neurons. Astrocytes were labeled for GLAST1, ALDH1L1 and GFAP. Microglia cells were labeled for IBA1, CD45 and TNFa. Neurons were labeled for NeuN, MAP2, TUJ1. B. Principal component analysis showing Kmeans clusters of DEGs in LPS and PAM3CSK4 related to control obtained from sample signals for each group. C-E. Volcano plots showing DEGs of isolated astrocytes(C), microglia (D), and neurons (E), respectively, in LPS related to control. F-H. Volcano plots showing DEGs in isolated astrocytes (F), microglia(G), and neurons (H), respectively, in PAM3CSK4 related to control. I-M. Enrichment analysis of top GOs in astrocytes, microglia and neurons either LPS related to Control or PAM3CSK4 related to control as indicated on top of each graph. N-O. Selected GOs enriched in neurons for neuronal and glial and metabolism processes. Note that we selected GOs of interest that were related to metabolism, neuronal processes and glial processes as indicated on the schematic. All volcano plots are represented with fold change > 2.0 and p-value < 0.05.

    Article Snippet: Then, GLAST1- cell suspension was incubated with CD11b MicroBeads UltraPure, mouse (Miltenyi, cat. 130–126-725) for positive selection of microglia.

    Techniques: Cell Isolation, Labeling, Control, Isolation